biotinylated pna Search Results


biotinylated pna  (Vector Laboratories)
95
Vector Laboratories biotinylated pna
Biotinylated Pna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated pna/product/Vector Laboratories
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biotinylated lotus tetragonolobus agglutinin  (Vector Laboratories)
93
Vector Laboratories biotinylated lotus tetragonolobus agglutinin
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Lotus Tetragonolobus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated lotus tetragonolobus agglutinin/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
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biotinylated peanut agglutinin or pna ba-2301-1 antibody  (EY Laboratories)
90
EY Laboratories biotinylated peanut agglutinin or pna ba-2301-1 antibody
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Peanut Agglutinin Or Pna Ba 2301 1 Antibody, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated peanut agglutinin or pna ba-2301-1 antibody/product/EY Laboratories
Average 90 stars, based on 1 article reviews
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5′-biotinyl pna-sl3-17  (PANAGENE Inc)
90
PANAGENE Inc 5′-biotinyl pna-sl3-17
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
5′ Biotinyl Pna Sl3 17, supplied by PANAGENE Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5′-biotinyl pna-sl3-17/product/PANAGENE Inc
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biotin-conjugated peanut agglutinin pna  (Seikagaku corporation)
90
Seikagaku corporation biotin-conjugated peanut agglutinin pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotin Conjugated Peanut Agglutinin Pna, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin-conjugated peanut agglutinin pna/product/Seikagaku corporation
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biotinylated γpna reporter probes  (PNA Innovations)
90
PNA Innovations biotinylated γpna reporter probes
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated γpna Reporter Probes, supplied by PNA Innovations, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated γpna reporter probes/product/PNA Innovations
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pna-biotin  (Seikagaku corporation)
90
Seikagaku corporation pna-biotin
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Pna Biotin, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated telomere-specific pna capture probes pctel-bio  (PCTel Inc)
90
PCTel Inc biotinylated telomere-specific pna capture probes pctel-bio
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Telomere Specific Pna Capture Probes Pctel Bio, supplied by PCTel Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated telomere-specific pna capture probes pctel-bio/product/PCTel Inc
Average 90 stars, based on 1 article reviews
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biotinylated lectin pna  (MBL Life science)
90
MBL Life science biotinylated lectin pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Lectin Pna, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated pna  (Biosearch Technologies Inc)
90
Biosearch Technologies Inc biotinylated pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Pna, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated pna/product/Biosearch Technologies Inc
Average 90 stars, based on 1 article reviews
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biotinylated pna  (BioTeZ Inc)
90
BioTeZ Inc biotinylated pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Pna, supplied by BioTeZ Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated pna/product/BioTeZ Inc
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biotinylated pna  (J-Oil Mills Inc)
90
J-Oil Mills Inc biotinylated pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Pna, supplied by J-Oil Mills Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated pna/product/J-Oil Mills Inc
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Image Search Results


Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus tetragonolobus agglutinin) + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.

Journal: Nature medicine

Article Title: Snail1-induced partial epithelial-to-mesenchymal transition drives renal fibrosis in mice and can be targeted to reverse established disease.

doi: 10.1038/nm.3901

Figure Lengend Snippet: Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus tetragonolobus agglutinin) + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.

Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies: biotinylated Lotus tetragonolobus agglutinin, LTA (B-1325, Vector Laboratories, Burlingame, CA); biotinylated peanut agglutinin, PNA (B-1075, Vector Laboratories, Burlingame, CA); α smooth muscle actin, (α-SMA) (Sigma-Aldrich, St Louis, MO); vimentin (sc-7557, Santa Cruz Biotechnology, Santa Cruz, CA); Snail1 (Ab1773; Abcam PLC, Cambridge, MA); phosphoNF-κB p65 (Ser276) (3037S, Cell Signaling Technology, Inc., Danvers, MA); phospho-Smad2 (Ser465/467) (3101S, Cell Signaling Technology, Inc., Danvers, MA); F4/80 (MCA497GA, Serotec, Oxford,UK); estrogen receptor alpha, ER-α (sc-543, Santa Cruz Biotechnology, Santa Cruz, CA); CD163 (sc-18796 Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Control, Staining, Expressing, MANN-WHITNEY, Immunolabeling, Immunofluorescence